General practitioners play a crucial role in implementing a One Health approach. This study investigates this implementation process between regulative framework and a selection of some practices adopted by general practitioners in relation to environmental health in Italy. Moreover, based on the literature review and on the support of Artificial Intelligence, it suggests a decalogue for the One Health general practitioners. The main challenges in the implementation process of One Health approach in primary care concern the investment in territorial health reform in Italy towards the House of the Communities, the valorization of the general practitioners practices related to the environmental health and the further testing and development of the recommended One Health General Decalogue.